The Penn Center for Molecular Discovery occupies 800 sq. ft.within the Institute for Medicine and Engineering (IME) within the Vagelos Research Building/Chemistry Complex on the campus of the University of Pennsylvania. The Institute for Medicine and Engineering (IME) at the University of Pennsylvania is a 10,000 sq. ft. laboratory facility that occupies the Roy and Diana Vagelos Research Building. The IME Laboratory Space, available to the PCMD, includes: 2 tissue culture rooms/6 hoods/8 incubators, a microscopy core, a microarraying core, cold rooms, radioisotope facilities, environmental rooms, autoclave and automated glassware washing. A conference room is located on the same floor and a 100 seat seminar room is located on the second floor of the Vagelos Building.
Resources within the PCMD include:
High Throughput Screening Line 1: 3-meter track/Thermo Catalyst 5 Robotic Arm (Polara Operating System); PerkinElmer Evolution EP3 (1536-pintool), Molecular Devices AquaMax 384/1536 multihead dispenser with 384/1536 washer, Thermo multidrop and microdrop (2); plate shaker, Biotek plate washer, PerkinElmer Janus 4-tip multiprobe, Envision multimode fluorescence plate reader, automated 189-plate automated incubator, barcode reader, Velocity plate sealer, Velocity robotic centrifuge, automated de-lidder, and automated plate carousel.
High Throughput Screening Line 2: PerkinElmer Evolution EP3 (384-pintool), Molecular Devices AquaMax 384/1536 multihead dispenser with 384/1536 washer, Envision multimode fluorescence plate reader, Thermo multidrop (2), Thermo FluoroSkan (2); Biomek 2000, Molecular Devices FlexStation III, EDC ATS-100 Acoustic Dispense; PerkinElmer 4-tip Multiprobe; COPAS Biosorters; Accuri C6 flow cytometer (96-well plate sampling).
High Content Imaging and Detection: Agilent 2-color microarray scanner; dual excitation/dual emission fluorimeter (SLM), whole plate scanning (Alpha Inotech; 15 um resolution scanner) and high resolution 2 um plate scanner (Alpha Inotech), Amersham Typhoon 4-color scanner/phosphorImager, GenePix 2-color microarray scanner, resonance light scanner, automated confocal scope with stage motion control (96/384 well plate).
Support Equipment: Matrix Wellmate/BSL2 hood; 3 C02 incubators; 4 -80C Freezers, 4 -20C Freezers; GeneMachine Omnigrid 100 and Accent microarrayer.
Bioproduction/Biopurification: 20x1L shakeflask incubator, rollerbottle cultivation. Multiple 1 and 2-L bioreactors (NBS); Six BSL2 tissue culture hoods and 12 CO2 incubators. Autoclave room.GE FPLC: ÄKTApurifier UPC 10 FPLC system with conductivity and 214, 256, and 280 nm UV detectors and Frac-950 fraction collector. Columns available for use include fast flow desalting columns for initial sample clarification (Hiprep 26/10, Hitrap desalting), fusion protein purification (Hisprep FF 16/10, Hitrap chelating, Hitrap streptavidin FF, Hitrap benzamidine FF columns), a high capacity size exclusion column for further purification (Superdex 75 pg Hiload 16/60), along with high-resolution ion exchange columns for final sample polishing (MonoQ/S 5/50 GL). LCMS: The high performance liquid chromatography (HPLC) unit consists of a binary high pressure pump (flowrates up to 10ml/min), autosampler (1.5ml HPLC vials or 96/384 well plate), and dual wavelength UV/VIS detector with a wavelength range of 190-700nm. This HPLC system is coupled to a high sensitivity Shimadzu LCMS-2010EV mass spectrometer with interchangeable ionization sources (electrospray (ESI) / atmospheric chemical (APCI)) capable of mass-to-charge ratio (m/z) detection from 10-2000 and a resolution of 2 mass units. The LC/MS system is attached to an automated fraction collector setup to collect analytical or preparative fractions based either the UV/VIS or mass detection signals.
Servers : The PCMD Informatics Core has operated a 7 TB server (Oracle) with tape backup. This consists of a linux-based IBM intellistation with 2 internal processors, and a 2-way tower server (x3500 7977 system) connected to the intellistation, complete with the CENTOS operating system. The server has 2 Dual-Core Xeon 5160 processors (3 GHz), 4 GB RAM, and a 2 x Gigabit Ethernet. Supported software includes: ActivityBase, SarGen, MOE, ADMET predictor.
Available to the PCMD, through the Central Spectroscopic Facilities of the Chemistry Department are: two high resolution mass spectrometers (VG 70/70 Micromass and ZAB) both interfaced to a dedicated mini-computer facility; two Bruker 200-MHz, two Bruker 250-MHz, and two Bruker 500-MHz NMR spectrometers, all equipped with Fourier transform (FT) capability; and two completely automated Kappa-Axis single crystal diffractometers. Multiple flow cytometers are available to the PCMD for an hourly rate through the Flow Cytometer Facility in the Department of Pathology at the University of Pennsylvania. Additionally, the Penn Proteomics Institute provides hourly rates for access to an numerous MALDI TOF/TOF and ESI mass spectrometers.