Three-dimensional flow focusing and cytometry (Issadore)

Purpose: The sensitive measurement of individual cells requires that each cell pass one-by-one through a small detection volume.This module employs fluidic structures to hydrodynamically focus cells laterally into the middle of a micro-channel, and chevron patterns to push cells vertically toward the bottom of the channel.(Howell, 2008).

Method of Fabrication/Use:Learn more

Microfluidic device for extreme shear rates and elongational flows (Diamond, SL)

Von Willebrand Factor fibers deposit on surfaces of collagen type 1 in a microfluidic model of coronary stenosis. (A-D) Platelet free plasma (5 mM EDTA) was perfused through a microfluidic channel over a collagen at the indicated wall shear rate (arrow, flow direction).  E, Computational fluid dynamics defined the wall shear rates in the outlet of the stenosis channel. F,  The colors indicate local wall shear rate and are equivalent to the scale bar in E.  G, The local wall shear rate along the central axis of the stenosis channel (dotted white line in F) indicates a steep gradient in shear rate (1,000 s-1 to 125,000 s-1) at the inlet and outlet.

Purpose: Microfluidic devices allow the generation of extreme shear rates useful for the study of thrombosis in severely stenosis coronary syndromes (Colace, 2013, Colace, 2013, Colace, 2012).  In assisted circulation pumps such as left ventricle assist devices (LVADs), extreme shear rates can cause depletion of von Willebrand factor (vWF), hemolysis, … Learn more

Microfluidic device for constant flowrate or constant transmural pressure gradients (Diamond, SL)

(A) Microfluidic that allows perfusion from an inlet (Q1) over a collagen plug held on a micropost array (B) to allow a constant pressure drop (and fluid permeation) across the collagen).  PDMS device shown at time of use with 3 pressure sensors and two infusion ports connected (C).

Purpose: Microfluidic devices allow the generation of flow fields and localized control of pressure drops across porous matrix or cellular assemblies (Muthard, 2012).  These devices have been used to measure endothelial migration and angiogenesis, blood clot permeability, clot retraction, and inner clot reaction dynamics (Muthard, 2013).  The design is especially … Learn more