Hydrodynamic Confined Microfluidic Probes (K. Turner)

Overview of hydrodynamic confined microfluidic (HCM) probe technology. (a) Schematic side-view of HCM probe.  A confined flow is created between the device and bottom surface of the well by flowing fluid into one port and removing it through the second port.  The gap height, h, is typically 20-60 m. (b) A schematic and photograph of an HCM device.  The ports are deep reactive ion etched into silicon and a PDMS cap is bonded to the backside of the device to allow fluidic connections to the port.  (c) Comparison of measured flow envelopes beneath the device imaged by flowing a fluorescent solution through the device (top) to CFD predictions of the flow.  Results shown for two different port geometries; all results are for a gap of 40 m.

Purpose: Microfluidic approaches have numerous applications in cell biology.  Microfluidics can be used to apply controlled hydrodynamic loads to cells, selectively treat cells with particular chemistries or drugs, and pattern proteins on surfaces.  The traditional channel-based microfluidics have broad applications and have been widely used, but in some experiments having … Learn more

Dielectrophoretic (DEP) Positioning and Sorting of Macromolecules and Nanoparticles (H. Bau)

Purpose: Sorting and positioning of objects in microfluidic channels such as cells, macromolecules, and micro/nano-particles is useful in a broad range of studies.  For example, positioning and holding a specific cell sitting in suspension via a dielectrophoretic trap can facilitate detailed inspection via microscopy or other interrogation techniques.  Furthermore, dielectrophoretic … Learn more

Micro-stirrers (mixers), Micro-Pumps, Valves for Flow Control (H. Bau)

Purpose: As the complexity of microfluidic experiments increases, it is desirable to integrate flow manipulation and control functions onto the chip.  Moving from off-chip control devices (e.g., syringe pumps) to on-chip systems allows for the complexity of devices to be increased and creates the opportunity for devices that can perform … Learn more