Microfluidic device for extreme shear rates and elongational flows (Diamond, SL)

Von Willebrand Factor fibers deposit on surfaces of collagen type 1 in a microfluidic model of coronary stenosis. (A-D) Platelet free plasma (5 mM EDTA) was perfused through a microfluidic channel over a collagen at the indicated wall shear rate (arrow, flow direction).  E, Computational fluid dynamics defined the wall shear rates in the outlet of the stenosis channel. F,  The colors indicate local wall shear rate and are equivalent to the scale bar in E.  G, The local wall shear rate along the central axis of the stenosis channel (dotted white line in F) indicates a steep gradient in shear rate (1,000 s-1 to 125,000 s-1) at the inlet and outlet.

Purpose: Microfluidic devices allow the generation of extreme shear rates useful for the study of thrombosis in severely stenosis coronary syndromes (Colace, 2013, Colace, 2013, Colace, 2012).  In assisted circulation pumps such as left ventricle assist devices (LVADs), extreme shear rates can cause depletion of von Willebrand factor (vWF), hemolysis, … Learn more