Muthard RW, Diamond SL. Side view thrombosis microfluidic device with controllable wall shear rate and transthrombus pressure gradient. Lab Chip May 21 2013; 13 (10): 1883-1891.
Hemodynamic conditions vary throughout the vasculature, creating diverse environments in which platelets must respond. To stop bleeding, a growing platelet deposit must … Learn more
Neeves KB, Maloney SF, Fong KP, Schmaier AA, Kahn ML, Brass LF, Diamond SL. Microfluidic focal thrombosis model for measuring murine platelet deposition and stability: PAR4 signaling enhances shear-resistance of platelet aggregates. J Thromb Haemost Dec 2008; 6 (12): 2193-2201.
Objective: To measure murine platelet deposition and stability in … Learn more
Welcome to the new web site for the Penn Biomedical Microfluidics Center.
Purpose: The delivery of drugs and/or genetic information across cell membranes while preserving the viability of the cells enables both scientific study of cellular mechanisms as well as the potential for genetic therapy. Such delivery can be accomplished by use of arrays of microneedles with tip sharpness on the nanoscale. … Learn more
Purpose: Microfluidic approaches have numerous applications in cell biology. Microfluidics can be used to apply controlled hydrodynamic loads to cells, selectively treat cells with particular chemistries or drugs, and pattern proteins on surfaces. The traditional channel-based microfluidics have broad applications and have been widely used, but in some experiments having … Learn more
Purpose: Rapid and accurate isolation of rare cells in fluid samples is a major challenge in medical diagnostics. There is a crucial need to isolate small numbers of cells from large populations, thus requiring high throughput techniques. The Issadore group at Penn has recently developed a new separation system to … Learn more
Purpose: A variety of techniques are available to measure the forces that cells exert as they are adhering or crawling. These techniques are known as traction force microscopies, and they include bead based traction microscopy (where beads are embedded in hydrogels) and microfabricated polymeric array detectors (or mPADs). The Chen … Learn more
Purpose: Sorting and positioning of objects in microfluidic channels such as cells, macromolecules, and micro/nano-particles is useful in a broad range of studies. For example, positioning and holding a specific cell sitting in suspension via a dielectrophoretic trap can facilitate detailed inspection via microscopy or other interrogation techniques. Furthermore, dielectrophoretic … Learn more
Purpose: As the complexity of microfluidic experiments increases, it is desirable to integrate flow manipulation and control functions onto the chip. Moving from off-chip control devices (e.g., syringe pumps) to on-chip systems allows for the complexity of devices to be increased and creates the opportunity for devices that can perform … Learn more
Purpose: There is an urgent need for new implantable devices to help understand and treat “brain network disorders.” For maximum research and clinical benefit, these implantable devices should allow signals to be recorded from clinically relevant volumes of brain, 100 cm2, at resolutions of 100 microns or less. Many of … Learn more